Prokaryotic相关论文
Specific T-cell Responses to CFP10,an Secreted Antigens of Mycobacterium Tuberculosis Protein,in Chi
Objective To construct prokaryotic expression vector of CFP-10 gene,and obtain recombinant protein,and the recombinant C......
采用RT-PCR方法分段扩增PB2基因,将目的基因定向克隆至含HisTAG的原核表达载体pET-32a,经序列验证正确后,转化BL21大肠杆菌,诱导表......
从牛布鲁菌基因组中克隆出VirB12基因,以pET-28α（＋）为模板构建VirB12原核表达载体pET-V12,pET-V12在E.coli BL21（DE3）中表达重组VirB12......
利用已分离的菌株HB070412,根据NCBI上GenBank中的HPS序列（ZP_02477753）设计了1对引物,用PCR方法扩增了副猪嗜血杆菌外膜蛋白中的穿......
Cloning, construction of prokaryotic expression vector and expression of Escherichia coli cytosine d
Cytosine 脱氨基酶基因 ofEscherichia coli 种类 H-30 被克隆，并且它鈥楪T G 鈥 ? 的开始 codon 被 PCR 变异到鈥楢T G 鈥 ? 。原......
Antimicrobial peptides are widely distributed in nature,existing in organisms of plants,insects,and vertebrates.It has b......
Gene cloning,expression analysis of JcACP (Acyl Carrier Protein) in Jatropha curcas L. and its proka
Objective To clone the ACP(acyl carrier protein)gene in Jatropha curcas L.,a potential anti-tumour and anti-fungal plant......
Construction of a recombinant plasmid pGEX-Sj26GST of Schistosoma japonicum and expression in Escher
Objective To construct and express a recombinant plasmid pGEX-Sj26GST of Schistosoma japonicum(Sj)in Escherichia coli(E.......
AIM:To express chimeric Fd (cFd) and chimeric light chain(cL) in E.coli respectively and refold them into chimericFab (......
Objective To construct a prokaryotic expression vector bearing fusion gene NT4-ADNF-9 for future studies on genetic ther......
CRISPR/Cas9 is a versatile genome-editing tool which is widely used for modifying the genome of both prokaryotic and euk......
Construction of hpaA gene from a clinical isolate of Helicobacter pyloriand identification of fusion
AIM:To clone hpaA gene from a clinical strain of Helicobacterpylori and to construct the expression vector of the genea......
AIM:To clone flagellin genes A(flaA)and B(flaB)from aclinical strain of Helicobacter pylori(H pylori)and to constructpr......
AIM:To clone and express the human colon mast cellcarboxypeptidase(MC-CP)gene.METHODS:Total RNA was extracted from colo......
Construction of prokaryotic expression system of ureB gene from a clinical Helicobacter pylori strai
AIM:To clone ureB gene from a clinical isolate ofHelicobacter pylori and construct a prokaryotic expressionsystem of th......
Construction of a prokaryotic expression system of vacA gene and detection of vacA gene, VacA protei
AIM:To construct a recombinant prokaryotic expressionvector inserted with Helicobacter pylori vacA gene andidentify the......
Construction of prokaryotic expression system of 2 148-bp fragment from cagA gene and detection of c
AIM:To construct a prokaryotic expression system of aHelicobacter py/ori ( H pylori) cagA gene fragment andestablish en......
AIM:To prepare and purify TAT-HBV targeted ribonucleasefusion protein,evaluate its transduction activity andinvestigate......
Prokaryotic expression of recombinant human p75NTR-Fc fusion protein and its effect on the neurite o
Objective: To clone, express, and identify the extracellular domain gene of human p75 neurotrophin receptor with IgG-Fc ......
AIM: To clone human liver special F protein and to express it in a prokaryotic system. METHODS: Total RNA was isolated f......
A Sensitive and Specific IgM-ELISA for the Serological Diagnosis of Human Leptospirosis Using a rLip
Objective To construct a lipL32/1‐lipL21‐OmpL1/2 fusion gene and its prokaryotic expression system,and to establish an......
Translation factor SelB is the key component for the specific decoding of UGA codons with selenocysteine at the ribosome......
AIM To study the epitope distribution of hepatitis G virus(HGV) and to seek for the potential recombinant antigensfor t......
The L protein (241 kD) of vesicular stomatitis virus (VSV) is the most important subunit of the replication complex. The......
Objective: To construct a prokaryotic recombinant vector for mouse lactate dehydrogenase-C and to detect its expression ......
Expression of Intracellular Domain of Epidermal Growth Factor Receptor and Generation of Its Monoclo
To prepare monoclonal antibody specific to epidermal growth factor receptor(EGFR)intracellular domain,itsgene was ampli......
Over-expression of 72 ku protein of wheat yellow mosaic virus in E. coli and preparation of its anti
By reverse transcription-polymerase chain reaction (RT-PCR), cDNA fragment of wheat yellow mosaic vir黶 (WYMV) RNA2 enc......
Disulfide-stabilized single-chain antibody-targeted superantigen: Construction of a prokaryotic expr
AIM: To construct the expression vector of B3 (scdsFv)-SEA (D227A) and to identify its binding and cytotoxic ability to ......
To determine the pathogenic potential of the leptospiral flagella-associated proteins, the genes flhA and flhB_2 encodin......
Construction of prokaryotic expression system of TGF-β1 epitope gene and identification of recombina
AIM: To insert the constructed TGF-β1 epitope gene into the el loop of C-terminus of truncated hepatitis B core antigen......
Protein expression and preparation of polydonal antibody of AD-004 and study on its expression in th
Objective To prepare rabbit antibody against mouse AD-004 by AD-004 expressed in the prokaryotic expression system and t......
Construction and prokaryotic expression of the fusion protein Stx2B-IntiminC300 of EHEC O157: H7 and
To construct and express the fusion protein Stx2B-IntiminC300 of EHEC 0157 :H7, and to further investigate its immunopro......
Establishment of method for seroepidermiological detection of human herpes virus 8 infection by usin
To establish a sensitive and specific method for seroepidermiological detection of human her- pes virus 8(HHV-8)infectio......
Preparation and Identification of HLA-A*1101 Tetramer Loading with Human Cytomegalovirus pp65 Antige
MHC/peptide tetramer technology has been widely used to study antigen-specific T cells, especially for identifying virus......
As an immediate-early protein of herpes simplex virus, infected-cell polypeptide 0 (ICP0) exhibits complicated interacti......
To purify the protein encoding the small capsid protein (SCP) of KSHV and analyze its immunogenicity, the carboxyl termi......
AIM: To construct a prokaryotic expression vector carrying Campylobacter jejuni peb1A gene and express it in Escherichia......
AIM: To investigate the ornithine decarboxylase (ODC) gene expression in colorectal carcinoma, ODC mRNA was assayed by R......
The ORFK8.1 of Kaposi’s sarcoma associated-herpesvirus(KSHV) was expressed in a prokaryotic expression system. The expr......
The cDNA encoding the G protein αq subunit was isolated from the antennae of Helicoverpa assulta (Guenée) by reverse t......
Prokaryotic Expression and Immunogenicity of Major Antigen Epitope of Rabbit Haemorrhagic Disease Vi
[Objective] To investigate the immunogenicity of main antigen epitope of RHDV (Rabbit haemorrhagic disease virus) VP60 e......
Prokaryotic Expression and Monoclonal Antibody Preparation of Chicken Major Histocompatibility Compl
[Objective] To prepare the monoclonal antibody against chicken major histocompatibility complex class II molecules(MHC I......
BACKGROUND:Studies have shown that the LRRN3,a member of the Neuron Leucine-Rich Repeat superfamily,could be related to ......
[Objective] The aim of the study was to obtain monoclonal antibody against chicken CD8 molecule. [Method] A fragment of ......
Objective: To facilitate manipulation of gene expression in different host cells, we used pEGFP-N1 as backbone to constr......
AIM:To identify and assess the novel makers for detection of Shiga toxin producing Escherichia coli (STEC) O157:H7 with ......
Aim:To explore the biofunctions of human B7-H4 generated from prokaryoticsystem.Methods:The gene of human B7-H4 extrace......
Phosphatase of regenerating liver 3(PRL3),which belongs to the superfamily of protein tyrosine phosphatases(PTPs),repres......
Objective To construct the prokaryotic expression vector p ET-32a(+)-C2orf69 and induce the expression of recombinant pr......
Objective To establish a reliable platform for screening glucokinase activators(GKAs)in vitro.Methods Pancreatic glucoki......
